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Title:
Main Experiment: Intensive sampling of enzyme activities of soil microbes under certain tree species (Site A)
Access rights:
Free within projects
Usage rights:
Data can be used by any member of the BEF-China project, or to others after consent is giving by SP13 (Francois Buscot and Jessica Gutknecht).
Published:
No information available
Abstract:
The soil microbial activity data from intensive sampling in site A in 2011. Activities of four hydrolytic enzymes involved in breakdown of cellulose (β-glucosidase), xylan(ß-xylosidase), chitin (N-acetyl-glucosaminidase) and polyphosphate (acid phosphatase) were measured by using methylumbelliferone substrate. Another two oxidative enzymes (phenol oxidase and peroxidase) which related to lignin degradation, their activities were analyzed by using substrate 3,3',5,5'-tetra:methyl-benzidine. The enzyme activities were measured on the plate reader (SynergyTM HT, Biotek) in xingangshan field lab.
Design:
Within each plot, three random trees of each tree species were selected for taking soil samples. For each targeted tree, we used soil core (10cm depth, diameter 6cm) took soils at four directions of the target tree on a distance of around 50cm from the tree trunk. Four soil cores of samples were homogenised and pooled into one sample in a plastic bag which represented the targeted tree. Soil samples were immediately sieved (2mm) in the field and preserved with liquid nitrogen.
Spatial extent:
Site A, Xingangshan
Temporal extent:
Soil samples were taken between October and November in 2011 in site A. Soil samples were freeze dried in the field lab andmeasured in the field lab after sampling campaign.
Taxonomic extent:
No information available
Measurement cirumstances:
No information available
Data analysis:
Enzyme activity was computed as µmol substrate converted per hour per g dry organic matter of sample

Filter:
Dataset column

Name:
tree-tag
Definition:
t tag of the respective individual in the field
Unit:
No information available
Datagroup:
t or s TAG Main Experiment
Keywords:
tree-tag
Values:
t103320010410
t103320010810
t103320010706
t103320010508
t103320010806
Contributors:
No information available

Dataset column

Name:
site
Definition:
site: Site A or B of the Main Experiment
Unit:
No information available
Datagroup:
Experimental Site
Keywords:
Site A
Values:
A
Contributors:
No information available

Dataset column

Name:
plot_code
Definition:
plot code
Unit:
No information available
Datagroup:
Plot name Main experiment (letter, number code)
Keywords:
plot
Values:
F21
E33
E34
C32
E31
Contributors:
No information available

Dataset column

Name:
species richness
Definition:
species richness level according to the design of the Main Experiment
Unit:
No information available
Datagroup:
Taxonomic biodiversity
Keywords:
species richness
Values:
1
8
16
4
2
Contributors:
No information available

Dataset column

Name:
species identity
Definition:
species identity, i.e. the species name
Unit:
No information available
Datagroup:
Scientific plant species name
Keywords:
species identity
Values:
Castanea henryi
Cyclobalanopsis glauca
Choerospondias axillaris
Castanopsis sclerophylla
Castanopsis eyrei
Contributors:
No information available

Dataset column

Name:
b-gluc
Definition:
enzyme activity of β-glucosidase
Unit:
nmol activity h-1 g soil
Datagroup:
Enzyme activity
Keywords:
hydrolytic enzyme
Values:
100.63510106188964
100.42022389702312
100.10946351592192
100.41629999904904
100.45478915788388
Contributors:

Dataset column

Name:
xyl
Definition:
enzyme activity of N-acetyl-glucosaminidase
Unit:
nmol activity h-1 g soil
Datagroup:
Enzyme activity
Keywords:
hydrolytic enzyme
Values:
100.09155453095504
14.710463874037917
106.02443802738321
112.87058671643484
16.05946347304276
Contributors:

Dataset column

Name:
ap
Definition:
enzyme activity of acid phosphatase
Unit:
nmol activity h-1 g soil
Datagroup:
Enzyme activity
Keywords:
hydrolytic enzyme
Values:
1006.0553295155527
1010.9087610559211
1013.2192745244073
1031.3678280096988
1017.4969523325747
Contributors:
No information available

Dataset column

Name:
nag
Definition:
enzyme activity of ß-xylosidase
Unit:
nmol activity h-1 g soil
Datagroup:
Enzyme activity
Keywords:
hydrolytic enzyme
Values:
101.26885854836706
102.50009818170298
100.71931447090824
102.34974314648791
100.28951261670136
Contributors:

No information available


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96 pei medium

Zhiqin
Pei

Owner of:
14 Datasets

Involved in:
2 Projects

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20 gutknecht medium

Jessica
Gutknecht

Owner of:
18 Datasets

Involved in:
2 Projects

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