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Title:
Main Experiment: Intensive sampling of enzyme activities of soil microbes under certain tree species (Site B)
Access rights:
Free within projects
Usage rights:
Data can be used by any member of the BEF-China project, or to others after consent is giving by SP13 (Francois Buscot and Jessica Gutknecht).
Published:
No information available
Abstract:
The soil microbial activity data from intensive sampling site B in 2012. Activities of four hydrolytic enzymes involved in breakdown of cellulose (β-glucosidase), xylan(ß-xylosidase), chitin (N-acetyl-glucosaminidase) and polyphosphate (acid phosphatase) were measured by using methylumbelliferone substrate. Another two oxidative enzymes (phenol oxidase and peroxidase) which related to lignin degradation, their activities were analyzed by using substrate 3,3',5,5'-tetra:methyl-benzidine. The enzyme activities were measured on the plate reader (SynergyTM HT, Biotek) in xingangshan field lab.
Design:
Within each plot, three random trees of each tree species were selected for taking soil samples. For each targeted tree, we used soil core (10cm depth, diameter 6cm) took soils at four directions of the target tree on a distance of around 50cm from the tree trunk. Four soil cores of samples were homogenised and pooled into one sample in a plastic bag which represented the targeted tree. Soil samples were immediately sieved (2mm) in the field and preserved with liquid nitrogen.
Spatial extent:
Study site, locations Site B, Xingangshan
Temporal extent:
Soil samples were taken between October and November in 2012 site B.Soil samples were freeze dried in the field lab and measured in the field lab after sampling campaign.
Taxonomic extent:
Range of organisms or individuals
Measurement cirumstances:
No information available
Data analysis:
Enzyme activity was computed as µmol substrate converted per hour per g dry organic matter of sample

Filter:
Dataset column

Name:
tree-tag
Definition:
t-tag of the tree accoring to the design of the main experiment
Unit:
No information available
Datagroup:
t or s TAG Main Experiment
Keywords:
tree-tag
Values:
t207280011407
t207280010612
t207280011313
t207280010515
t207280011312
Contributors:
No information available

Dataset column

Name:
site
Definition:
site
Unit:
No information available
Datagroup:
Experimental Site
Keywords:
Site B
Values:
B
Contributors:
No information available

Dataset column

Name:
plot_code
Definition:
plot code
Unit:
No information available
Datagroup:
Plot name Main experiment (letter, number code)
Keywords:
plot
Values:
J29
M24
I25
G28
M22
Contributors:
No information available

Dataset column

Name:
species richness
Definition:
species diversity level according to the design of the Main Experiment
Unit:
No information available
Datagroup:
Taxonomic biodiversity
Keywords:
species richness
Values:
1
2
4
8
16
Contributors:
No information available

Dataset column

Name:
species identity
Definition:
species taxonomic name
Unit:
No information available
Datagroup:
Scientific plant species name
Keywords:
species identity
Values:
Ailanthus altissima
Alniphyllum fortunei
Castanopsis fargesii
Betula luminifera
Celtis biondii
Contributors:
No information available

Dataset column

Name:
b-gluc
Definition:
enzyme activity of β-glucosidase
Unit:
nmol activity h-1 g soil
Datagroup:
Enzyme activity
Keywords:
hydrolytic enzyme
Values:
100.87729923751998
100.00728667220557
101.34195921516759
101.71791280420491
103.03022971763303
Contributors:

Dataset column

Name:
xyl
Definition:
enzyme activity of N-acetyl-glucosaminidase
Unit:
nmol activity h-1 g soil
Datagroup:
Enzyme activity
Keywords:
hydrolytic enzyme
Values:
100.26382393722967
105.9016526421704
105.65974597873277
105.5149472790449
109.26473122803856
Contributors:

Dataset column

Name:
ap
Definition:
enzyme activity of acid phosphatase
Unit:
nmol activity h-1 g soil
Datagroup:
Enzyme activity
Keywords:
hydrolytic enzyme
Values:
1019.9575135485159
1032.043545903418
1016.4262886272172
1044.3318038666475
1036.6747588570338
Contributors:
No information available

Dataset column

Name:
nag
Definition:
enzyme activity of ß-xylosidase
Unit:
nmol activity h-1 g soil
Datagroup:
Enzyme activity
Keywords:
hydrolytic enzyme
Values:
10.857245056713511
100.13086424826464
10.3224007656419
107.1469261524294
102.80775671991486
Contributors:

No information available


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96 pei medium

Zhiqin
Pei

Owner of:
14 Datasets

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2 Projects

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20 gutknecht medium

Jessica
Gutknecht

Owner of:
18 Datasets

Involved in:
2 Projects

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